Anti-Mouse Akt1 (N-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Mouse
Host Species	Rabbit
Accession Number	P31750
Gene Id	11651
Gene Symbols	AKT1
Protein Name / Synonyms	Akt1 (N-term)
Clonality	Polyclonal
Preparation	This Mouse Akt1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 22-49 amino acids from the N-terminal region of mouse Akt1.
Concentration (lot specific)	0.5 mg/ml
Recommended Applications	Western Blotting, IHC-P
Research Area	Angiogenesis, Apoptosis, Cardiovascular Disease, Autophagy
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

This gene encodes the founding member of the Akt serine-threonine protein kinase gene family that also includes Akt2 and Akt3. This kinase is a major downstream effector of the phosphatidylinositol 3-kinase (PI3K) pathway that mediates the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). It is activated through recruitment to cellular membranes by PI3K lipid products and by phosphorylation by 3-phosphoinositide dependent kinase-1. It then further phosphorylates different downstream proteins in response to various extracellular signals and thus plays a pivotal role in mediating a variety of cellular processes, such as glucose metabolism, glycogen biosynthesis, protein synthesis and turn over, inflammatory response, cell survival (anti-apoptosis) and development. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].

Specificity

Predicted Reactivity

Human

Other Names

Akt1; Akt; Rac; RAC-alpha serine/threonine-protein kinase; AKT1 kinase; Protein kinase B; Protein kinase B alpha; Proto-oncogene c-Akt; RAC-PK-alpha; Thymoma viral proto-oncogene

NCBI Accession #

NP_001159366.1;NP_033782.1

Other Accession #

P31749; NP_033782.1; XP_001479205.1

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

55707

Recommended Dilutions

WB: 1:1000; IHC: 1:10-50

	Mouse Akt1 Antibody (N-term) (Cat# 102-12612) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the Akt1 antibody detected the Akt1 protein (arrow).
	Mouse Akt1 Antibody (N-term) (102-12612)immunohistochemistry analysis in formalin fixed and paraffin embedded mouse gallbladder tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Mouse Akt1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Antigen Source

MOUSE

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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