Anti-mouse CASP3 (N-term S12) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Mouse
Host Species	Rabbit
Accession Number	P70677\|NP_033940.1
Gene Id	12367
Gene Symbols	Casp3
Protein Name / Synonyms	Caspase-3, CASP-3, Apopain, Cysteine protease CPP32, CPP-32, LICE, Protein Yama, SREBP cleavage activity 1, SCA-1, Caspase-3 subunit p17, Caspase-3 subunit p12, Casp3, Cpp32
Clonality	Polyclonal
Clone	RB39712
Isotype	Kappa, Rabbit
Recommended Applications	Western Blotting
Research Area	Cardiovascular Disease
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

CASP3 is involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9 (By similarity). Cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes.

Other Information

NCBI Accession #

NP_033940.1

Antigen Type

Synthetic Peptide

Format

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Calculated Molecular Weight (Da)

31475

Antigen Source

Mouse

Images

WB (1:1000)

Anti-CASP3 Antibody at 1:1000 dilution + mouse spleen lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 31 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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