Anti-Mouse CD4 Antibody, Purified

CD4 (cluster of differentiation 4), also known as L3T4, is a 55 kDa glycoprotein . It is a member of the Ig superfamily and is primarily expressed on T helper cells, on regulatory T cells, at lower levels on subpopulations of NKT cells, some populations of dendritic cells, and on thymocytes (CD4+CD8+ and CD4+CD8€“ thymocytes). CD4 acts as a coreceptor with the TCR during T cell activation and thymic differentiation by binding MHC class II and associating with the protein tyrosine kinase, lck. This antibody is widely used to detect mouse CD4 T cells and to deplete CD4 T cells in vivo.

Each lot of antibody has been tested by flow cytometry staining. We suggest use ≤ 0.5 µg per million cells in 100 µl volume of staining buffer. Researchers are recommended to further titrate this antibody at the optimal concentration for each specific application.

Keep the lyophilized antibodies at -20 °C. Once reconstituted with PBS, store at 2 °C - 8 °C for less than 3 weeks or freeze at -20°C for long term storage. Please limit freeze and thaw cycles.