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Anti-Mouse CD40LG Antibody
Catalog #: 144-00327
CD40LG Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Accession Number | P29965 |
| Gene Id | 959 |
| Gene Symbols | CD40LG |
| Protein Name / Synonyms | CD40LG, CD154, CD40L, HIGM1, IGM, IMD3, T-BAM, TNFSF5, TRAP, gp39, hCD40L, CD40 ligand |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IF |
| Research Area | Cardiovascular Disease, Inflammation |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
The protein encoded by this gene is expressed on the surface of T cells. It regulates B cell function by engaging CD40 on the B cell surface. A defect in this gene results in an inability to undergo immunoglobulin class switch and is associated with hyper-IgM syndrome.
Dilutions
WB 1:500 - 1:2000
IF 1:50 - 1:200
IF 1:50 - 1:200
Format
Calculated MW
29kDa
Observed MW
30kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 47-223 of human CD40LG (NP_000065.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using CD40LG antibody (144-00327) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Immunofluorescence analysis of U2OS cells using CD40LG antibody (144-00327). Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.