Anti-Mouse CD44 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	P16070
Gene Id	960
Gene Symbols	CD44
Protein Name / Synonyms	CD44, CDW44, CSPG8, ECMR-III, HCELL, HUTCH-I, IN, LHR, MC56, MDU2, MDU3, MIC4, Pgp1, CD44 antigen
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC, IF
Research Area	Cardiovascular Disease, Inflammation
Shipping Type	Blue ice
Storage	-20°C

Description

Background

The protein encoded by this gene is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). This protein participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200

Format

Calculated MW

3kDa/15kDa/32-53kDa/73-81kDa

Observed MW

82kDa

Immunogen

Recombinant protein of human CD44

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of mouse spleen, using CD44 Antibody (144-012410) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Western blot - [KO Validated] CD44 Polyclonal Antibody (144-012410)

Western blot analysis of extracts from normal (control) and CD44 knockout (KO) HeLa cells, using CD44 antibody (144-012410) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.
Western blot - [KO Validated] CD44 Polyclonal Antibody (144-012410)

Immunohistochemistry of paraffin-embedded rat bronchus using CD44 antibody (144-012410) at dilution of 1:100 (40x lens).
Immunohistochemistry - [KO Validated] CD44 Polyclonal Antibody (144-012410)

Immunofluorescence analysis of HeLa cells using CD44 antibody (144-012410) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence - [KO Validated] CD44 Polyclonal Antibody (144-012410)

Expiration:

12 months from the date of shipment when stored properly.

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