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Anti-Mouse CD44 Antibody, RayBright® Red 780
Catalog #: 135-27015
Mouse CD44 Antibody [clone: KM114], monoclonal rat IgG1 kappa. Validated for flow cytometry.
Product Description
Specifications
| Size | 25 µg, 100 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Mouse |
| Host Species | Rat |
| Protein Name / Synonyms | CD44, H-CAM, HUTCH and lymphocyte antigen 24 (Ly-24) |
| Clonality | Monoclonal |
| Clone | KM114 |
| Conjugation | RayBright® Red 780 |
| Preparation | The antibody was purified by affinity chromatography. |
| Isotype | IgG1 Kappa |
| Format | Liquid |
| Concentration (lot specific) | 0.5 mg/ml |
| Buffers | PBS, pH 7.2-7.4, 0.2% BSA, 0.1% sodium azide |
| Recommended Applications | Flow Cytometry |
| Research Area | Cardiovascular Disease |
| Excitation Laser | Red Laser, 633 nm |
| Maximum Emission Wavelength | 780 nm, replace APC-Alexa Fluor™ 750 tandems |
| Shipping Type | Blue ice |
| Storage | 4°C |
Description
Product Features
- Serum-free media used for cell culture of hybridomas to minimize bovine antibody contamination
- Gentle elution at pH 4.0 to minimize antibody degradation
- Protein A, G & L affinity purification
- High purity
Description
CD44 is an 85-90kD glycoprotein which is expressed on all leukocytes, endothelial cells, hepatocytes, and mesenchymal cells. CD44 expression can change between high, medium, and low levels depending on if B or T cells have been activated or progressed to the memory stage. CD44 is associated with leukocyte attachment to endothelial cells and leukocyte aggregation.
Recommended Usage
Each lot of antibody has been tested by flow cytometry staining. We suggest use ≤ 0.5 µg per 106 cells in 100 µl volume of staining buffer. Researchers are recommended to further titrate this antibody at the optimal concentration for each specific application.
Storage / Stability
Store at 2 °C ~ 8 °C, protected from prolonged light exposure. DO NOT FREEZE.