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Anti-Mouse CD54 Antibody, PE
Catalog #: 135-18058
Mouse CD54 Antibody [clone: BE29G1], monoclonal rat IgG2a. Validated for flow cytometry.
Product Description
Specifications
| Size | 25 µg, 100 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Mouse |
| Host Species | Rat |
| Protein Name / Synonyms | CD54, ICAM-1 |
| Clonality | Monoclonal |
| Clone | BE29G1 |
| Conjugation | PE |
| Preparation | The antibody was purified by affinity chromatography. |
| Isotype | IgG2a |
| Format | Liquid |
| Concentration (lot specific) | 0.5 mg/ml |
| Buffers | PBS, pH 7.2-7.4, 0.2% BSA, 0.1% sodium azide |
| Recommended Applications | Flow Cytometry |
| Research Area | Cardiovascular Disease |
| Excitation Laser | Blue Laser, 488 nm / Green Laser, 561 nm |
| Maximum Emission Wavelength | 575 nm |
| Shipping Type | Blue ice |
| Storage | 4°C |
Description
Product Features
- Serum-free media used for cell culture of hybridomas to minimize bovine antibody contamination
- Gentle elution at pH 4.0 to minimize antibody degradation
- Protein A, G & L affinity purification
- High purity
Description
CD54, also known as ICAM1, is a 9-kD a immunoglobulin superfamily member. CD54 is expressed on lymphocytes, high endothelial venules (HEV), dendritic cells, and lymphocytes. CD54 is a ligand for LFA1 (CD11a/CD18) and Mac-1 (CD11b/CD18) and plays an important role in intracellular adhesion of T cells and B cells.
Recommended Usage
Each lot of antibody has been tested by flow cytometry staining. We suggest use ≤ 0.5 µg per million cells in 100 µl volume of staining buffer. Researchers are recommended to further titrate this antibody at the optimal concentration for each specific application.
Storage / Stability
Store at 2 °C ~ 8 °C, protected from prolonged light exposure. DO NOT FREEZE.