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Anti-Mouse CD71 Antibody, Purified
Catalog #: 135-00059
Mouse CD71 Antibody [clone: R17217.1.3], monoclonal Rat IgG2a. Validated for flow cytometry.
Product Description
Specifications
| Size | 100 µg, 500 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Mouse |
| Host Species | Rat |
| Protein Name / Synonyms | Transferrin receptor |
| Clonality | Monoclonal |
| Clone | R17217.1.3 |
| Preparation | The antibody was purified by affinity chromatography. |
| Isotype | IgG2a |
| Format | Lyophilized |
| Concentration (lot specific) | Lyophilized |
| Buffers | PBS, pH 7.2-7.4; Carrier-free |
| Recommended Applications | Flow Cytometry |
| Reconstitution | Reconstitute in desired amount of PBS. |
| Research Area | Cardiovascular Disease |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Product Features
- Serum-free media used for cell culture of hybridomas to minimize bovine antibody contamination
- Gentle elution at pH 4.0 to minimize antibody degradation
- Protein A, G & L affinity purification
- High purity
- Carrier free
- Endotoxin-free
Description
CD71 is a type II transmembrane protein with two disulfide-linked monomers. It is also known as the transferrin receptor. CD71 is expressed on proliferating cells and developing erythroids. CD71 mediates the iron uptake in cells to control cellular proliferation.
Recommended Usage
Each lot of antibody has been tested by flow cytometry staining. We suggest use ≤ 0.5 µg per million cells in 100 µl volume of staining buffer. Researchers are recommended to further titrate this antibody at the optimal concentration for each specific application.
Storage / Stability
Keep the lyophilized antibodies at -20 °C. Once reconstituted with PBS, store at 2 °C - 8 °C for less than 3 weeks or freeze at -20°C for long term storage. Please limit freeze and thaw cycles.