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Anti-Mouse CD86 Antibody, Purified
Catalog #: 135-00041
Mouse CD86 Antibody [clone: GL1], monoclonal Rat IgG2a kappa. Validated for flow cytometry.
Product Description
Specifications
| Size | 100 µg, 500 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Mouse |
| Host Species | Rat |
| Accession Number | P42082 |
| Gene Symbols | Cd86 |
| Protein Name / Synonyms | CD86, B7.2, B70, Ly-58 |
| Clonality | Monoclonal |
| Clone | GL1 |
| Preparation | The antibody was purified by affinity chromatography. |
| Isotype | IgG2a Kappa |
| Format | Lyophilized |
| Concentration (lot specific) | Lyophilized |
| Buffers | PBS, pH 7.2-7.4; Carrier-free |
| Recommended Applications | Flow Cytometry |
| Reconstitution | Reconstitute in desired amount of PBS. |
| Research Area | Cardiovascular Disease |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Product Features
- Serum-free media used for cell culture of hybridomas to minimize bovine antibody contamination
- Gentle elution at pH 4.0 to minimize antibody degradation
- Protein A, G & L affinity purification
- High purity
- Carrier free
- Endotoxin-free
Description
CD86 is an 80 kD Ig superfamily member also known as B7.2. CD86 is expressed on mature dendritic cells, macrophages, astrocytes and activated B cells and T cells. As costimulatory factors, CD86 and CD80, interact with CD28 and CD152 (CTLA-4) to regulate T cell activation, with CD28 transducing activation signal, and CTLA4 transducing inhibitory signal. CD86 has also been shown to be involved in immunoglobulin class-switching and triggering of NK cell-mediated cytotoxicity.
Recommended Usage
Each lot of antibody has been tested by flow cytometry staining. We suggest use ≤ 0.5 µg per million cells in 100 µl volume of staining buffer. Researchers are recommended to further titrate this antibody at the optimal concentration for each specific application.
Storage / Stability
Keep the lyophilized antibodies at -20 °C. Once reconstituted with PBS, store at 2 °C - 8 °C for less than 3 weeks or freeze at -20°C for long term storage. Please limit freeze and thaw cycles.