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Anti-Mouse CD8a Antibody, RayBright® Blue 488
Catalog #: 135-14026
RayBright® Blue 488 Mouse CD8a Antibody [clone: 53-6.72], monoclonal Rat IgG2a kappa. Validated for flow cytometry.
Product Description
Specifications
| Size | 25 µg, 100 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Mouse |
| Host Species | Rat |
| Gene Symbols | CD8A |
| Protein Name / Synonyms | CD8a |
| Clonality | Monoclonal |
| Clone | 53-6.72 |
| Conjugation | RayBright® Blue 488 |
| Preparation | The antibody was purified by affinity chromatography, and conjugated with a high quality fluorochrome, RayBright® Blue 488 |
| Isotype | IgG2a Kappa |
| Format | Liquid |
| Concentration (lot specific) | 0.5 mg/ml |
| Buffers | PBS, pH 7.2-7.4, 0.2% BSA, 0.1% sodium azide |
| Recommended Applications | Flow Cytometry |
| Research Area | Cardiovascular Disease |
| Excitation Laser | Blue Laser, 488 nm |
| Maximum Emission Wavelength | 518 nm, replace FITC, Alexa Fluor™ 488, and BD BB515 (Alexa Fluor™ is the trademark of Invitrogen) |
| Shipping Type | Blue ice |
| Storage | 4°C |
Description
Product Features
- Serum-free media used for cell culture of hybridomas to minimize bovine antibody contamination
- Gentle elution at pH 4.0 to minimize antibody degradation
- Protein A, G & L affinity purification
- High purity
Typical Data
Description
CD8 (cluster of differentiation 8), also known as Lyt-2, Ly-2, is a surface glycoprotein comprising of α and β chains and is primarily expressed on immature thymocytes, mature CD8 T cells and some other cell types, e.g., dendritic cells. CD8 exist as either α/β heterodimer in most thymocytes or α/α homodimer on a subset ofγ/δ TCR-bearing intraepithelial lymphocytes. CD8 is an antigen co-receptor on T cells that associates with the T cell receptor complex during antigen presentation through MHC class I by APC cells. This antibody recognizes the 34 kDa CD8 α chain on the cell surface.
Product Details
Other Names
Lyt-2, Ly-2
Recommended Usage
Each lot of antibody has been tested by flow cytometry staining. We suggest use ≤ 0.5 µg per 106 cells in 100 µl volume of staining buffer. Researchers are recommended to further titrate this antibody at the optimal concentration for each specific application.
Storage/Stability
Stored at 2°C ~ 8°C, protected from prolonged light exposure. DO NOT FREEZE.