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Anti-Mouse Dnmt1 (Center) Antibody
Catalog #: 102-24311
Rabbit Anti-(Mouse) Dnmt1 (Center) Antibody, 400 µl
Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Accession Number | P13864 |
| Gene Id | 13433 |
| Gene Symbols | Dnmt1 |
| Protein Name / Synonyms | DNA (cytosine-5)-methyltransferase 1, Dnmt1, Met-1, DNA methyltransferase MmuI, DNA MTase MmuI, MMmuI, MCMT, Dnmt1, Dnmt, Met1, Uim |
| Clonality | Polyclonal |
| Clone | RB51461 |
| Isotype | Kappa, Rabbit |
| Recommended Applications | IF, Western Blotting, IHC-P |
| Research Area | Cardiovascular Disease |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Introduction
Methylates CpG residues. Preferentially methylates hemimethylated DNA. Associates with DNA replication sites in S phase maintaining the methylation pattern in the newly synthesized strand, that is essential for epigenetic inheritance. Associates with chromatin during G2 and M phases to maintain DNA methylation independently of replication. It is responsible for maintaining methylation patterns established in development. DNA methylation is coordinated with methylation of histones. Mediates transcriptional repression by direct binding to HDAC2. In association with DNMT3B and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9.
Other Information
Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Calculated Molecular Weight (Da)
183189
Antigen Source
Human
Images
IF (1:50)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling Dnmt1 with 102-24311 at 1/50 dilution, followed by Dylight® 488-conjugated goat anti-rabbit IgG (NK179883) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing nucleus staining on HeLa cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin (PD18466410) at 1/100 dilution (red).
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.