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Anti-Mouse F4/80 Ag. Antibody, FITC
Catalog #: DS-MB-03907
Rat Anti-Mouse F4/80 Ag. [+FITC]
Product Description
Specifications
| Size | 50 µg |
| Estimated Lead Time | 1-2 weeks |
| Species | Mouse |
| Specificity | The F4/80 antibody recognizes the murine F4/80 antigen, a 160 kD cell surface glycoprotein that is a member of the EGF-TM7 family of proteins which shares 68% overall amino acid identity with human EGF module-containing mucin-like hormone receptor 1 (EMR1). Expression of F4/80 is heterogeneous and is reported to vary during macrophage maturation and activation. The F4/80 antigen is expressed on a wide range of mature tissue macrophages including Kupffer cells, Langerhans, microglia, macrophages located in the gut lamina propria, peritoneal cavity, lung, thymus, bone marrow stroma and macrophages in the red pulp of the spleen. F4/80 expression has also been reported on a subpopulation of dendritic cells but is absent from macrophages located in T cell areas of the spleen and lymph node. The ligands and biological functions of the F4/80 antigen have not yet been determined but recent studies suggest a role for F4/80 in the generation of efferent CD8+ve regulatory T cells. |
| Protein Name / Synonyms | F4/80 Ag. |
| Concentration (lot specific) | IgG concentration 0.1 mg/ml |
| Recommended Applications | Flow Cytometry, Immunofluorescence |
| Research Area | Cardiovascular Disease |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Storage/Stability
Store at +4 °C or at -20 °C if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 18 months after receipt.
Format
Supplied as:
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid.
Formulation:
Phosphate buffered saline
Preparation
Immunogen:
Thioglycollate stimulated peritoneal macrophages from C57/BL mice
Preservative:
| 0.09% | Sodium Azide |
| 1% | Bovine Serum Albumin |
This product
Spleen cells from immunised HOB2 rats were fused with cells of the mouse NS-1 myeloma cell line.
Expiration:
12 months from the date of shipment when stored properly.