Anti-Mouse GABARAPL2 Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P60520
Gene Id	11345
Gene Symbols	GABARAPL2
Protein Name / Synonyms	GABARAPL2
Clonality	Polyclonal
Preparation	This GABARAPL2 antibody is generated from rabbits immunized with human GABARAPL2 recombinant protein.
Concentration (lot specific)	0.250 mg/ml
Recommended Applications	Western Blotting, IHC-P, FC
Research Area	Neuroscience, Cardiovascular Disease, Autophagy
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Membrane proteins located on vesicles (v-SNAREs) and on the target membrane (t-SNAREs) mediate specific recognition and, possibly, fusion between a transport vesicle and its target membrane. The activity of SNARE molecules is regulated by several soluble cytosolic proteins. We have cloned a bovine brain cDNA encoding a conserved 117 amino acid polypeptide, denoted Golgi-associated ATPase Enhancer of 16 kDa (GATE-16), that functions as a soluble transport factor. GATE-16 interacts with N-ethylmaleimidesensitive factor (NSF) and significantly stimulates its ATPase activity. It also interacts with the Golgi v-SNARE GOS-28 in an NSF-dependent manner. We propose that GATE-16 modulates intra-Golgi transport through coupling between NSF activity and SNAREs activation.

Specificity

Other Names

GABARAPL2; FLC3A; GEF2; Gamma-aminobutyric acid receptor-associated protein-like 2; GABA(A) receptor-associated protein-like 2; Ganglioside expression factor 2; General protein transport factor p16; Golgi-associated ATPase enhancer of 16 kDa; MAP1 light chain 3-related protein

NCBI Accession #

NP_009216.1

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

13667

Recommended Dilutions

WB: 1:1000; IHC: 1:50-100; FC: 1:10-50

	Western blot analysis of GABARAPL2 Antibody (Cat# 102-13739) in MCF-7 cell line lysates (35ug/lane). GABARAPL2 (arrow) was detected using the purified Pab.
	Formalin-fixed and paraffin-embedded mouse brain tissue reacted with GABARAPL2 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
	GABARAPL2 Antibody (Cat# 102-13739) FC analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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