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Anti-Mouse GATA3(M) (190-209aa) Antibody
Catalog #: 130-10557
Rabbit Anti-Mouse GATA3(M) (190-209aa) Antibody
Product Description
Specifications
| Size | 20 µg, 100 µg, 200 µg, 500 µg, 1 mg |
| Estimated Lead Time | 1-2 business days |
| Species | Mouse |
| Host Species | Rabbit |
| Accession Number | P23772 |
| Gene Id | 14462 |
| Gene Symbols | Gata3 Gata-3 |
| Protein Name / Synonyms | Trans-acting T-cell-specific transcription factor GATA-3 (GATA-binding factor 3) |
| Clonality | Polyclonal |
| Preparation | This antibody was produced from antiserum of rabbits immunized with a synthetic immunogen GATA3(M) (190-209aa). This antibody was produced from a rabbit immunized with this peptide conjugated with KLH. The IgG fraction was purified by Protein A affinity chromatography. |
| Recommended Applications | ELISA (recommended work dilution= 1:1, 000) |
| Reconstitution | Product is supplied as a powder obtained from lyophilization of purified antibody in glycine solution. Reconstitute the antibody with sterile water to a final concentration of 1 mg/ml. |
| Research Area | Cardiovascular Disease |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Description
The genes for all 4 subunits of the T-cell antigen receptor (alpha, beta, gamma and delta) are controlled by distinct enhancers and their enhancer-binding proteins. Marine and Winoto (1991) identified a common TCR regulatory element by demonstrating binding of the enhancer-binding protein GATA3 to the enhancer elements of all 4 TCR genes. GATA3 had been shown in the chicken to be an enhancer-binding protein containing a zinc finger domain. GATA3 mRNA was demonstrated by Northern blot analysis in T cells but not in B cells or macrophages. GATA3 is abundantly expressed in the T-lymphocyte lineage and is thought to participate in T-cell receptor gene activation through binding to enhancers. Labastie et al. (1994) cloned the human gene and the 5-prime end of the mouse gene. The human gene comprises 6 exons distributed over 17 kb of DNA. Its 2 zinc fingers are encoded by 2 separate exons highly conserved with those of GATA1, but no other structural homologies between the 2 genes could be found.
Storage/Stability
Store at -20°C. If intended for use within a month, may keep at 4°C. Minimize freeze-thaw cycles.
Expiration
12 months from the date of shipment when stored properly.