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Anti-Mouse I-Ab and I-Ad Antibody, RayBright® Red 647
Catalog #: 135-24068
Mouse I-Ab and I-Ad Antibody [clone: BP107.2.2], monoclonal mouse IgG3. Validated for flow cytometry.
Product Description
Specifications
| Size | 25 µg, 100 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Mouse |
| Host Species | Mouse |
| Clonality | Monoclonal |
| Clone | BP107.2.2 |
| Conjugation | RayBright® Red 647 |
| Preparation | The antibody was purified by affinity chromatography. |
| Isotype | IgG3 |
| Format | Liquid |
| Concentration (lot specific) | 0.5 mg/ml |
| Buffers | PBS, pH 7.2-7.4, 0.2% BSA, 0.1% sodium azide |
| Recommended Applications | Flow Cytometry |
| Research Area | Cardiovascular Disease |
| Excitation Laser | Red Laser, 633 nm |
| Maximum Emission Wavelength | 664 nm, replace Cy5™ and Alexa Fluor™ 647 (Cy5™ and Alexa Fluor™ are the trademark of Invitrogen and GE Health Care) |
| Shipping Type | Blue ice |
| Storage | 4°C |
Description
Product Features
- Serum-free media used for cell culture of hybridomas to minimize bovine antibody contamination
- Gentle elution at pH 4.0 to minimize antibody degradation
- Protein A, G & L affinity purification
- High purity
Description
I-Ab and IAd are mouse MHC class II allogantigens expressed in mice of H-2b and H2-d halptypes. Mouse MHC class II molecules are expressed on antigen presenting cells, including dendritic cells, macrophages, monocytes and B cells. This antibody recognizes I-Ab, I-Ad, I-Aq, I-Ed, and I-Ek from mouse H2b, d, q and k haplotypes. MHC class II interacts with T cell receptor complex and present antigenic peptide to activate CD4 T cells.
Recommended Usage
Each lot of antibody has been tested by flow cytometry staining. We suggest use ≤ 0.5 µg per 106 cells in 100 µl volume of staining buffer. Researchers are recommended to further titrate this antibody at the optimal concentration for each specific application.
Storage / Stability
Store at 2 °C ~ 8 °C, protected from prolonged light exposure. DO NOT FREEZE.