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Anti-Mouse IFN-gamma Antibody, RayBright® Red 780
Catalog #: 135-27029
Mouse IFN-gamma Antibody [clone: R4-6A2], monoclonal rat IgG1 kappa. Validated for flow cytometry.
Product Description
Specifications
| Size | 25 µg, 100 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Mouse |
| Host Species | Rat |
| Gene Symbols | IFNG |
| Clonality | Monoclonal |
| Clone | R4-6A2 |
| Conjugation | RayBright® Red 780 |
| Preparation | The antibody was purified by affinity chromatography. |
| Isotype | IgG1 Kappa |
| Format | Liquid |
| Concentration (lot specific) | 0.5 mg/ml |
| Buffers | PBS, pH 7.2-7.4, 0.2% BSA, 0.1% sodium azide |
| Recommended Applications | Flow Cytometry |
| Research Area | Cardiovascular Disease |
| Excitation Laser | Red Laser, 633 nm |
| Maximum Emission Wavelength | 780 nm, replace APC-Alexa Fluor™ 750 tandems |
| Shipping Type | Blue ice |
| Storage | 4°C |
Description
Product Features
- Serum-free media used for cell culture of hybridomas to minimize bovine antibody contamination
- Gentle elution at pH 4.0 to minimize antibody degradation
- Protein A, G & L affinity purification
- High purity
Description
Interferon-gamma (IFNγ) is a dimerized soluble cytokine and the only member of the type II class of interferons. IFNγ plays a critical role in innate and adaptive immunity against viral, some bacterial, and protozoal infections. IFNγ activates macrophages, induces MHC class II expression, targets virus-infected cells, and inhibits viral replication directly. IFNγ can also regulate the functions of many immune cells, including T cells and macrophages. IFNγ is produced by activated CD4 (Th1) cells,CD8 CTLs, NK, and NKT cells. IFN-γ can also upregulate MHC class I and II antigen expression in antigen-presenting cells
Recommended Usage
Each lot of antibody has been tested by flow cytometry staining. We suggest use ≤ 0.5 µg per million cells in 100 µl volume of staining buffer. Researchers are recommended to further titrate this antibody at the optimal concentration for each specific application.
Storage / Stability
Store at 2 °C ~ 8 °C, protected from prolonged light exposure. DO NOT FREEZE.