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Anti-Mouse MHC class II Antibody, Purified
Catalog #: 135-00036
Mouse MHC class II Antibody [clone: M5/114.15.2], monoclonal Rat IgG2b kappa. Validated for flow cytometry.
Product Description
Specifications
| Size | 100 µg, 500 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Mouse |
| Host Species | Rat |
| Protein Name / Synonyms | MHC class II |
| Clonality | Monoclonal |
| Clone | M5/114.15.2 |
| Preparation | The antibody was purified by affinity chromatography. |
| Isotype | IgG2b Kappa |
| Format | Lyophilized |
| Concentration (lot specific) | Lyophilized |
| Buffers | PBS, pH 7.2-7.4; Carrier-free |
| Recommended Applications | Flow Cytometry |
| Reconstitution | Reconstitute in desired amount of PBS. |
| Research Area | Cardiovascular Disease |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Product Features
- Serum-free media used for cell culture of hybridomas to minimize bovine antibody contamination
- Gentle elution at pH 4.0 to minimize antibody degradation
- Protein A, G & L affinity purification
- High purity
- Carrier free
- Endotoxin-free
Description
Mouse MHC class II molecules are expressed on antigen presenting cells, including dendritic cells, macrophages, monocytes and B cells. This antibody recognizes I-Ab, I-Ad, I-Aq, I-Ed, and I-Ek from mouse H2b, d, q and k haplotypes. MHC class II interacts with T cell receptor complex and present antigenic peptide to activate CD4 T cells.
Recommended Usage
Each lot of antibody has been tested by flow cytometry staining. We suggest use ≤ 0.5 µg per million cells in 100 µl volume of staining buffer. Researchers are recommended to further titrate this antibody at the optimal concentration for each specific application.
Storage / Stability
Keep the lyophilized antibodies at -20 °C. Once reconstituted with PBS, store at 2 °C - 8 °C for less than 3 weeks or freeze at -20°C for long term storage. Please limit freeze and thaw cycles.