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Anti-Mouse NK1.1 Antibody, Purified
Catalog #: 135-00038
Mouse NK1.1 Antibody [clone: PK136], monoclonal Rat IgG2a kappa. Validated for flow cytometry.
Product Description
Specifications
| Size | 100 µg, 500 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Mouse |
| Host Species | Rat |
| Protein Name / Synonyms | KLRB1 |
| Clonality | Monoclonal |
| Clone | PK136 |
| Preparation | The antibody was purified by affinity chromatography. |
| Isotype | IgG2a Kappa |
| Format | Lyophilized |
| Concentration (lot specific) | Lyophilized |
| Buffers | PBS, pH 7.2-7.4; Carrier-free |
| Recommended Applications | Flow Cytometry |
| Reconstitution | Reconstitute in desired amount of PBS. |
| Research Area | Cardiovascular Disease |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Product Features
- Serum-free media used for cell culture of hybridomas to minimize bovine antibody contamination
- Gentle elution at pH 4.0 to minimize antibody degradation
- Protein A, G & L affinity purification
- High purity
- Carrier free
- Endotoxin-free
Description
NK1.1 surface antigen is encoded by the NKR-P1B/NKR-P1C gene. Also named KLRB1 protein, NKR-P1A or CD161 is classified as a type II membrane protein. NK1.1 is expressed by natural killer cells and a subset of T cells in the mouse strains C57BL/6, FVB/N, and NZB but is not present in the mouse strains AKR, BALB/c, CBA/J, C3H, DBA/1, DBA/2, NOD, SJL, and 129. NK cells play an important role in anti-tumor immunity and allograft rejection after bone marrow transplantation. Dendritic cells present tumor antigen to NK cells through CD1d molecules.
Recommended Usage
Each lot of antibody has been tested by flow cytometry staining. We suggest use ≤ 0.5 µg per million cells in 100 µl volume of staining buffer. Researchers are recommended to further titrate this antibody at the optimal concentration for each specific application.
Storage / Stability
Keep the lyophilized antibodies at -20 °C. Once reconstituted with PBS, store at 2 °C - 8 °C for less than 3 weeks or freeze at -20°C for long term storage. Please limit freeze and thaw cycles.