Anti-Mouse/Rat ATP2A2 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Mouse, Rat
Host Species	Rabbit
Accession Number	P16615
Gene Id	488
Gene Symbols	ATP2A2
Protein Name / Synonyms	ATP2A2, ATP2B, DAR, DD, SERCA2, sarcoplasmic/endoplasmic reticulum calcium ATPase 2
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC
Research Area	Cardiovascular Disease, Autophagy
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes one of the SERCA Ca(2+)-ATPases, which are intracellular pumps located in the sarcoplasmic or endoplasmic reticula of muscle cells. This enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol into the sarcoplasmic reticulum lumen, and is involved in regulation of the contraction/relaxation cycle. Mutations in this gene cause Darier-White disease, also known as keratosis follicularis, an autosomal dominant skin disorder characterized by loss of adhesion between epidermal cells and abnormal keratinization. Alternative splicing results in multiple transcript variants encoding different isoforms.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:100

Format

Calculated MW

109kDa/111kDa/114kDa

Observed MW

115kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 111-253 of human ATP2A2 (NP_733765.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using ATP2A2 antibody (144-00098) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Western blot - ATP2A2 Polyclonal Antibody (144-00098)

Expiration:

12 months from the date of shipment when stored properly.

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