Anti-Mouse/Rat IKBKB Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	O14920
Gene Id	3551
Gene Symbols	IKBKB
Protein Name / Synonyms	IKBKB, IKK-beta, IKK2, IKKB, IMD15, NFKBIKB, inhibitor of nuclear factor kappa-B kinase subunit beta
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IF
Research Area	Cardiovascular Disease, Cancer, Inflammation
Shipping Type	Blue ice
Storage	-20°C

Description

Background

The protein encoded by this gene phosphorylates the inhibitor in the inhibitor/NF-kappa-B complex, causing dissociation of the inhibitor and activation of NF-kappa-B. The encoded protein itself is found in a complex of proteins. Several transcript variants, some protein-coding and some not, have been found for this gene.

Dilutions

WB 1:500 - 1:2000
IF 1:50 - 1:200

Format

Calculated MW

29kDa/79kDa/85kDa/86kDa

Observed MW

86kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 517-756 of human IKBKB (NP_001547.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using IKBKB antibody (144-00714) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.
Western blot - IKBKB Polyclonal Antibody (144-00714)

Immunofluorescence analysis of U2OS cells using IKBKB antibody (144-00714) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence - IKBKB Polyclonal Antibody (144-00714)

Expiration:

12 months from the date of shipment when stored properly.

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