MAP1LC3A Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q9H492 |
| Gene Id | 84557 |
| Gene Symbols | MAP1LC3A |
| Protein Name / Synonyms | MAP1LC3A, ATG8E, LC3, LC3A, MAP1ALC3, MAP1BLC3, microtubule-associated proteins 1A/1B light chain 3A |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Research Area | Cardiovascular Disease, Autophagy |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. The protein encoded by this gene is one of the light chain subunits and can associate with either MAP1A or MAP1B. Two transcript variants encoding different isoforms have been found for this gene. The expression of variant 1 is suppressed in many tumor cell lines, suggesting that may be involved in carcinogenesis.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IHC 1:50 - 1:200
Format
Calculated MW
14kDa
Observed MW
18kDa
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human MAP1LC3A (NP_115903.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using MAP1LC3A antibody (144-011438) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat ovary using MAP1LC3A antibody (144-011438) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using MAP1LC3A antibody (144-011438) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse testis using MAP1LC3A antibody (144-011438) at dilution of 1:100 (20x lens).
Immunohistochemistry of paraffin-embedded mouse spinal cord using MAP1LC3A antibody (144-011438) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using MAP1LC3A antibody (144-011438) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.