Anti-Mouse/Rat PARK2 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	O60260
Gene Id	5071
Gene Symbols	PARK2
Protein Name / Synonyms	PRKN, AR-JP, LPRS2, PARK2, PDJ, E3 ubiquitin-protein ligase parkin
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IF
Research Area	Cardiovascular Disease, Cancer, Autophagy
Shipping Type	Blue ice
Storage	-20°C

Description

Background

The precise function of this gene is unknown; however, the encoded protein is a component of a multiprotein E3 ubiquitin ligase complex that mediates the targeting of substrate proteins for proteasomal degradation. Mutations in this gene are known to cause Parkinson disease and autosomal recessive juvenile Parkinson disease. Alternative splicing of this gene produces multiple transcript variants encoding distinct isoforms. Additional splice variants of this gene have been described but currently lack transcript support.

Dilutions

WB 1:500 - 1:2000
IF 1:50 - 1:200

Format

Calculated MW

23kDa/30-51kDa

Observed MW

52kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human PARK2 (AAH22014.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using PARK2 antibody (144-00968) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Western blot - PARK2 Polyclonal Antibody (144-00968)

Immunofluorescence analysis of HeLa cells using PARK2 antibody (144-00968).
Immunofluorescence - PARK2 Polyclonal Antibody (144-00968)

Expiration:

12 months from the date of shipment when stored properly.

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