Anti-Mouse/Rat TNRC6A Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	Q8NDV7
Gene Id	27327
Gene Symbols	TNRC6A
Protein Name / Synonyms	TNRC6A, CAGH26, GW1, GW182, TNRC6, trinucleotide repeat containing 6A
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IF
Research Area	Cardiovascular Disease
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes a member of the trinucleotide repeat containing 6 protein family. The protein functions in post-transcriptional gene silencing through the RNA interference (RNAi) and microRNA pathways. The protein associates with messenger RNAs and Argonaute proteins in cytoplasmic bodies known as GW-bodies or P-bodies. Inhibiting expression of this gene delocalizes other GW-body proteins and impairs RNAi and microRNA-induced gene silencing.

Dilutions

WB 1:500 - 1:1000
IF 1:50 - 1:100

Format

Calculated MW

17kDa/36kDa/182kDa/205kDa/206kDa/210kDa

Observed MW

180kDa

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 950-1050 of human TNRC6A (NP_055309.2).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using TNRC6A antibody (144-06115) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Western blot - TNRC6A antibody (144-06115)

Immunofluorescence analysis of U2OS cells using TNRC6A antibody (144-06115) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence - TNRC6A antibody (144-06115)

Expiration:

12 months from the date of shipment when stored properly.

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