Anti-MPI Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	P34949
Gene Id	4351
Gene Symbols	MPI
Protein Name / Synonyms	MPI, CDG1B, PMI, PMI1, mannose phosphate isomerase
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC, IF
Shipping Type	Blue ice
Storage	-20°C

Description

Background

Phosphomannose isomerase catalyzes the interconversion of fructose-6-phosphate and mannose-6-phosphate and plays a critical role in maintaining the supply of D-mannose derivatives, which are required for most glycosylation reactions. Mutations in the MPI gene were found in patients with carbohydrate-deficient glycoprotein syndrome, type Ib. Alternative splicing results in multiple transcript variants.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:100

Format

Calculated MW

39kDa/46kDa

Observed MW

42kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-423 of human MPI (NP_002426.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using MPI antibody (144-07319) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Western blot - MPI Polyclonal Antibody (144-07319)

Immunohistochemistry of paraffin-embedded rat heart using MPI antibody (144-07319) at dilution of 1:100 (40x lens).
Immunohistochemistry - MPI Polyclonal Antibody (144-07319)

Immunofluorescence analysis of MCF7 cells using MPI antibody (144-07319). Blue: DAPI for nuclear staining.
Immunofluorescence - MPI Polyclonal Antibody (144-07319)

Expiration:

12 months from the date of shipment when stored properly.

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