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Anti-MRPS30 Antibody
Catalog #: 144-05841
MRPS30 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q9NP92 |
| Gene Id | 10884 |
| Gene Symbols | MRPS30 |
| Protein Name / Synonyms | MRPS30, MRP-S30, PAP, PDCD9, S30mt, mitochondrial ribosomal protein S30 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF, IP |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that is similar to the chicken pro-apoptotic protein p52. Transcript variants using alternative promoters or polyA sites have been mentioned in the literature but the complete description of these sequences is not available.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:100
IP 1:50 - 1:200
IHC 1:50 - 1:200
IF 1:50 - 1:100
IP 1:50 - 1:200
Format
Calculated MW
50kDa
Observed MW
54kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 190-439 of human MRPS30 (NP_057724.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using MRPS30 antibody (144-05841) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat lung using MRPS30 Antibody (144-05841) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using MRPS30 Antibody (144-05841) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using MRPS30 antibody (144-05841). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200ug extracts of HepG2 cells using 1ug MRPS30 antibody (144-05841). Western blot was performed from the immunoprecipitate using MRPS30 antibody (144-05841) at a dilition of 1:1000.
Expiration:
12 months from the date of shipment when stored properly.