Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	P43246
Gene Id	4436
Gene Symbols	MSH2
Protein Name / Synonyms	MSH2, COCA1, FCC1, HNPCC, HNPCC1, LCFS2, mutS homolog 2
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC, IP
Research Area	Cancer
Shipping Type	Blue ice
Storage	-20°C

Background

This locus is frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). When cloned, it was discovered to be a human homolog of the E. coli mismatch repair gene mutS, consistent with the characteristic alterations in microsatellite sequences (RER+ phenotype) found in HNPCC. Two transcript variants encoding different isoforms have been found for this gene.

Dilutions

WB 1:500 - 1:2000
IHC 1:50- 1:200
IP 1:20 - 1:50

Format

Calculated MW

97kDa/104kDa

Observed MW

105kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human MSH2 (NP_000242.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using MSH2 antibody (144-01121) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.
Western blot - MSH2 Polyclonal Antibody (144-01121)

Immunoprecipitation analysis of 150ug extracts of A549 cells using 3ug MSH2 antibody (144-01121). Western blot was performed from the immunoprecipitate using MSH2 antibody (144-01121) at a dilition of 1:1000.
Immunoprecipitation - MSH2 Polyclonal Antibody (144-01121)