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Anti-MSRB1 Antibody
Catalog #: 144-06737
MSRB1 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q9NZV6 |
| Gene Id | 51734 |
| Gene Symbols | MSRB1 |
| Protein Name / Synonyms | MSRB1, HSPC270, SELR, SELX, SEPX1, SepR, methionine sulfoxide reductase B1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
This gene encodes a selenoprotein, which contains a selenocysteine (Sec) residue at its active site. The selenocysteine is encoded by the UGA codon that normally signals translation termination. The 3' UTR of selenoprotein genes have a common stem-loop structure, the sec insertion sequence (SECIS), that is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. This protein belongs to the methionine sulfoxide reductase (Msr) protein family which includes repair enzymes that reduce oxidized methionine residues in proteins. The protein encoded by this gene is expressed in a variety of adult and fetal tissues and localizes to the cell nucleus and cytosol.
Dilutions
WB 1:500 - 1:2000
IF 1:50 - 1:100
IF 1:50 - 1:100
Format
Calculated MW
12kDa
Observed MW
13kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-94 of human MSRB1 (NP_057416.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using MSRB1 Antibody (144-06737) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Immunofluorescence analysis of HeLa cells using MSRB1 antibody (144-06737). Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.