Anti-MTAP Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	Q13126
Gene Id	4507
Gene Symbols	MTAP
Protein Name / Synonyms	MTAP, BDMF, DMSFH, DMSMFH, HEL-249, LGMBF, MSAP, c86fus, methylthioadenosine phosphorylase
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC, IF
Research Area	Cancer
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes an enzyme that plays a major role in polyamine metabolism and is important for the salvage of both adenine and methionine. The encoded enzyme is deficient in many cancers because this gene and the tumor suppressor p16 gene are co-deleted. Multiple alternatively spliced transcript variants have been described for this gene, but their full-length natures remain unknown.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200

Format

Calculated MW

26kDa/30kDa/31kDa/32kDa/33kDa/36kDa/38kDa

Observed MW

31kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-283 of human MTAP (NP_002442.2).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using MTAP antibody (144-01049) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Western blot - MTAP Polyclonal Antibody (144-01049)

Immunohistochemistry of paraffin-embedded rat brain using MTAP antibody (144-01049) at dilution of 1:100 (40x lens).
Immunohistochemistry - MTAP Polyclonal Antibody (144-01049)

Immunohistochemistry of paraffin-embedded mouse liver using MTAP antibody (144-01049) at dilution of 1:100 (40x lens).
Immunohistochemistry - MTAP Polyclonal Antibody (144-01049)

Immunofluorescence analysis of A-549 cells using MTAP antibody (144-01049). Blue: DAPI for nuclear staining.
Immunofluorescence - MTAP Polyclonal Antibody (144-01049)

Expiration:

12 months from the date of shipment when stored properly.

Featured Products