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Anti-Muscle Actin Antibody, Sodium Azide Free
Catalog #: 188-10207-SAF
Mouse anti-Human/Mouse/Rabbit/Rat Muscle Actin Antibody [Sodium Azide Free]
Product Description
Specifications
| Size | 100 µg |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Specificity | Other species not known |
| Host Species | Mouse |
| Accession Number | P68133 |
| Gene Id | 58 |
| Gene Symbols | ACTA1,ACTA |
| Protein Name / Synonyms | Actin, alpha skeletal muscle (Alpha-actin-1) |
| Immunogen | SDS extract of human myocardium was used as the immunogen for this Muscle actin HHF35 antibody. |
| Isotype | Kappa, IgG1 |
| Purity | Protein G affinity chromatography |
| Concentration (lot specific) | 1 mg/ml in 1X PBS (pH 7.4), BSA free, sodium azide free |
| Recommended Applications | Western blot: 0.5-1ug/ml FACS:0.5-1ug/million cells IF: 0.5-1ug/ml IHC (FFPE): 0.5-1.0ug/ml for 30 minutes at RT (1) Prediluted format: incubate for 30 min at RT (2) |
| Domain / Region / Terminus | Cytoplasmic |
| Research Area | Cytoskeletal Reorganization |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
Description
Description
This antibody recognizes actin of skeletal, cardiac, and smooth muscle cells. It is not reactive with other mesenchymal cells except for myoepithelium. Actin can be resolved on the basis of its isoelectric points into three distinctive components: alpha, beta, and gamma in order of increasing isoelectric point. This mAb recognizes alpha and gamma isotypes of all muscle groups. Nonmuscle cells such as vascular endothelial cells and connective tissues are non-reactive. Also, neoplastic cells of nonmuscle-derived tissue such as carcinomas, melanomas, and lymphomas are negative. It stains tumors of smooth (leiomyomas and leiomyosarcomas) as well as skeletal muscle (rhabdomyomas and rhabdomyosarcomas).
Storage
Store the Muscle Actin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Expiration:
12 months from the date of shipment when stored properly.