Anti-MYCN Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	P04198
Gene Id	4613
Gene Symbols	MYCN
Protein Name / Synonyms	MYCN, MODED, N-myc, NMYC, ODED, bHLHe37, N-myc proto-oncogene protein
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC
Research Area	Cancer
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene is a member of the MYC family and encodes a protein with a basic helix-loop-helix (bHLH) domain. This protein is located in the nucleus and must dimerize with another bHLH protein in order to bind DNA. Amplification of this gene is associated with a variety of tumors, most notably neuroblastomas. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200

Format

Calculated MW

49kDa

Observed MW

45kDa

Immunogen

Recombinant protein of human MYCN

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using MYCN antibody (144-00499) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Western blot - MYCN Polyclonal Antibody (144-00499)

Immunohistochemistry of paraffin-embedded mouse brain using MYCN antibody (144-00499) (40x lens).
Immunohistochemistry - MYCN Polyclonal Antibody (144-00499)

Immunohistochemistry of paraffin-embedded rat brain using MYCN antibody (144-00499) (40x lens).
Immunohistochemistry - MYCN Polyclonal Antibody (144-00499)

Expiration:

12 months from the date of shipment when stored properly.

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