Anti-MYH1 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	P12882
Gene Id	4619
Gene Symbols	MYH1
Protein Name / Synonyms	MYH1, HEL71, MYHSA1, MYHa, MyHC-2X/D, MyHC-2x, myosin-1
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC, IF
Shipping Type	Blue ice
Storage	-20°C

Description

Background

Myosin is a major contractile protein which converts chemical energy into mechanical energy through the hydrolysis of ATP. Myosin is a hexameric protein composed of a pair of myosin heavy chains (MYH) and two pairs of nonidentical light chains. Myosin heavy chains are encoded by a multigene family. In mammals at least 10 different myosin heavy chain (MYH) isoforms have been described from striated, smooth, and nonmuscle cells. These isoforms show expression that is spatially and temporally regulated during development.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:100

Format

Calculated MW

223kDa

Observed MW

260kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human MYH1 (NP_005954.3).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using MYH1 antibody (144-06935) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.
Western blot - MYH1 antibody (144-06935)

Immunohistochemistry of paraffin-embedded rat brain using MYH1 Antibody (144-06935) at dilution of 1:100 (40x lens).
Immunohistochemistry - MYH1 Antibody (144-06935)

Immunofluorescence analysis of U2OS cells using MYH1 antibody (144-06935). Blue: DAPI for nuclear staining.
Immunofluorescence - MYH1 antibody (144-06935)

Expiration:

12 months from the date of shipment when stored properly.

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