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Anti-MYH10 Antibody
Catalog #: 144-12029
MYH10 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P35580 |
| Gene Id | 4628 |
| Gene Symbols | MYH10 |
| Protein Name / Synonyms | MYH10, NMMHC-IIB, NMMHCB, myosin-10 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Research Area | Cardiovascular Disease |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
This gene encodes a member of the myosin superfamily. The protein represents a conventional non-muscle myosin; it should not be confused with the unconventional myosin-10 (MYO10). Myosins are actin-dependent motor proteins with diverse functions including regulation of cytokinesis, cell motility, and cell polarity. Mutations in this gene have been associated with May-Hegglin anomaly and developmental defects in brain and heart. Multiple transcript variants encoding different isoforms have been found for this gene.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IHC 1:50 - 1:200
Format
Calculated MW
228kDa/230kDa/231kDa/232kDa
Observed MW
250kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1908-2007 of human MYH10 (NP_001242941.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using MYH10 antibody (144-012029) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Immunohistochemistry of paraffin-embedded rat brain using MYH10 antibody (144-012029) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using MYH10 antibody (144-012029) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat testis using MYH10 antibody (144-012029) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse testis using MYH10 antibody (144-012029) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.