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Anti-MYH7 Antibody
Catalog #: 144-07564
MYH7 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P12883 |
| Gene Id | 4625 |
| Gene Symbols | MYH7 |
| Protein Name / Synonyms | MYH7, CMD1S, CMH1, MPD1, MYHCB, SPMD, SPMM, myosin-7 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Concentration (lot specific) | 2.36 mg/ml |
| Buffers | PBS with 0.05% proclin300, 50% glycerol, pH 7.3 |
| Recommended Applications | Western Blotting, IHC |
| Research Area | Cardiovascular Disease |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
Muscle myosin is a hexameric protein containing 2 heavy chain subunits, 2 alkali light chain subunits, and 2 regulatory light chain subunits. This gene encodes the beta (or slow) heavy chain subunit of cardiac myosin. It is expressed predominantly in normal human ventricle. It is also expressed in skeletal muscle tissues rich in slow-twitch type I muscle fibers. Changes in the relative abundance of this protein and the alpha (or fast) heavy subunit of cardiac myosin correlate with the contractile velocity of cardiac muscle. Its expression is also altered during thyroid hormone depletion and hemodynamic overloading. Mutations in this gene are associated with familial hypertrophic cardiomyopathy, myosin storage myopathy, dilated cardiomyopathy, and Laing early-onset distal myopathy.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:100
IHC 1:50 - 1:100
Format
Calculated MW
223kDa
Observed MW
223kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1766-1935 of human MYH7 (NP_000248.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using MYH7 antibody (144-07564) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.
Immunohistochemistry of paraffin-embedded mouse lung using MYH7 antibody (144-07564) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.