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Anti-MYOD1 Antibody
Catalog #: 144-00671
MYOD1 Polyclonal Antibody
Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Accession Number | P15172 |
| Gene Id | 4654 |
| Gene Symbols | MYOD1 |
| Protein Name / Synonyms | MYOD1, MYF3, MYOD, PUM, bHLHc1, myogenic differentiation 1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, pH7.3 |
| Recommended Applications | Western Blotting, IF |
| Research Area | Cancer |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
This gene encodes a nuclear protein that belongs to the basic helix-loop-helix family of transcription factors and the myogenic factors subfamily. It regulates muscle cell differentiation by inducing cell cycle arrest, a prerequisite for myogenic initiation. The protein is also involved in muscle regeneration. It activates its own transcription which may stabilize commitment to myogenesis.
Dilutions
WB 1:500 - 1:1000
IF 1:20 - 1:50
IF 1:20 - 1:50
Format
Calculated MW
34kDa
Observed MW
45kDa
Immunogen
A synthetic peptide of human MYOD1
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using MYOD1 antibody (144-00671) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Expiration:
12 months from the date of shipment when stored properly.