Anti-MYOF Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	Q9NZM1
Gene Id	26509
Gene Symbols	MYOF
Protein Name / Synonyms	MYOF, FER1L3, myoferlin
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting
Shipping Type	Blue ice
Storage	-20°C

Description

Background

Mutations in dysferlin, a protein associated with the plasma membrane, can cause muscle weakness that affects both proximal and distal muscles. The protein encoded by this gene is a type II membrane protein that is structurally similar to dysferlin. It is a member of the ferlin family and associates with both plasma and nuclear membranes. The protein contains C2 domains that play a role in calcium-mediated membrane fusion events, suggesting that it may be involved in membrane regeneration and repair. Two transcript variants encoding different isoforms have been found for this gene. Other possible variants have been detected, but their full-length nature has not been determined.

Dilutions

WB 1:200 - 1:2000

Format

Calculated MW

17kDa/46kDa/49kDa/179kDa/229kDa/233kDa/234kDa

Observed MW

250kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-190 of human MYOF (NP_038479.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using MYOF antibody (144-61049) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 2s.
Western blot - MYOF antibody (144-61049)

Expiration:

12 months from the date of shipment when stored properly.

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