This mAb recognizes both the free and protein-conjugated (either soluble or cell bound) form of fluorescein or carboxy-fluorescein. Sensitivity of the FITC-anti-FITC system is reportedly similar to that of the biotin-avidin system. In biotin or enzyme-labeled form, this mAb is also suitable for the conversion of fluorescein-labeled detection into immuno-enzymatic assays.

Store the FITC antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).