3607 Parkway Ln Ste 200 Norcross, Georgia 30092
www.RayBiotech.com | info@raybiotech.com
770-729-2992 | +1-888-494-8555
ISO 13485:2016 cGMP
Anti-NAMPT Antibody
Catalog #: 144-00256
NAMPT Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P43490 |
| Gene Id | 10135 |
| Gene Symbols | NAMPT |
| Protein Name / Synonyms | NAMPT, 1110035O14Rik, PBEF, PBEF1, VF, VISFATIN, nicotinamide phosphoribosyltransferase |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Research Area | Inflammation |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
This gene encodes a protein that catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, one step in the biosynthesis of nicotinamide adenine dinucleotide. The protein belongs to the nicotinic acid phosphoribosyltransferase (NAPRTase) family and is thought to be involved in many important biological processes, including metabolism, stress response and aging. This gene has a pseudogene on chromosome 10.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IHC 1:50 - 1:200
IF 1:50 - 1:200
Format
Calculated MW
55kDa
Observed MW
56kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human NAMPT (NP_005737.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using NAMPT antibody (144-00256) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat liver using NAMPT antibody (144-00256) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat ovary using NAMPT antibody (144-00256) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat spleen using NAMPT antibody (144-00256) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human tonsil using NAMPT antibody (144-00256) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon carcinoma using NAMPT antibody (144-00256) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spleen using NAMPT antibody (144-00256) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using NAMPT antibody (144-00256).
Expiration:
12 months from the date of shipment when stored properly.