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Anti-NANOG Antibody
Catalog #: 144-03232
NANOG Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q9H9S0 |
| Gene Id | 79923 |
| Gene Symbols | NANOG |
| Protein Name / Synonyms | NANOG, Nanog homeobox |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
The protein encoded by this gene is a DNA binding homeobox transcription factor involved in embryonic stem (ES) cell proliferation, renewal, and pluripotency. The encoded protein can block ES cell differentiation and can also autorepress its own expression in differentiating cells. Two transcript variants encoding different isoforms have been found for this gene.
Dilutions
WB 1:1000 - 1:2000
Format
Calculated MW
32kDa/34kDa
Observed MW
48kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human NANOG (NP_079141.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using NANOG antibody (144-03232) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 45s.
Expiration:
12 months from the date of shipment when stored properly.