Anti-NBN Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	O60934
Gene Id	4683
Gene Symbols	NBN
Protein Name / Synonyms	NBN, AT-V1, AT-V2, ATV, NBS, NBS1, P95, nibrin
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting
Research Area	Cancer
Shipping Type	Blue ice
Storage	-20°C

Description

Background

Mutations in this gene are associated with Nijmegen breakage syndrome, an autosomal recessive chromosomal instability syndrome characterized by microcephaly, growth retardation, immunodeficiency, and cancer predisposition. The encoded protein is a member of the MRE11/RAD50 double-strand break repair complex which consists of 5 proteins. This gene product is thought to be involved in DNA double-strand break repair and DNA damage-induced checkpoint activation.

Dilutions

WB 1:500 - 1:1000

Format

Calculated MW

84kDa

Observed MW

110kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 435-754 of human NBN (NP_002476.2).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using NBN antibody (144-60031) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Western blot - [KO Validated] NBN Polyclonal Antibody (144-60031)

Western blot analysis of extracts from normal (control) and NBN knockout (KO) HeLa cells, using NBN antibody (144-60031) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.
Western blot - [KO Validated] NBN Polyclonal Antibody (144-60031)

Expiration:

12 months from the date of shipment when stored properly.

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