Anti-NCAM1 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	P13591
Gene Id	4684
Gene Symbols	NCAM1
Protein Name / Synonyms	NCAM1, CD56, MSK39, NCAM, neural cell adhesion molecule 1
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.05% proclin300, 50% glycerol, pH 7.3
Recommended Applications	Western Blotting, IHC
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes a cell adhesion protein which is a member of the immunoglobulin superfamily. The encoded protein is involved in cell-to-cell interactions as well as cell-matrix interactions during development and differentiation. The encoded protein has been shown to be involved in development of the nervous system, and for cells involved in the expansion of T cells and dendritic cells which play an important role in immune surveillance. Alternative splicing results in multiple transcript variants.

Dilutions

WB 1:500 - 1:2000
IHC 1:100 - 1:200

Format

Calculated MW

40kDa/73kDa/80kDa/83kDa/93kDa/94kDa

Observed MW

140kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 759-858 of human NCAM1 (NP_851996.2).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using NCAM1 antibody (144-07913) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Western blot - NCAM1 antibody (144-07913)

Immunohistochemistry of paraffin-embedded human stomach using NCAM1 antibody (144-07913) at dilution of 1:100 (40x lens).
Immunohistochemistry - NCAM1 antibody (144-07913)

Expiration:

12 months from the date of shipment when stored properly.

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