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Anti-NCBP2 Antibody
Catalog #: 144-07293
NCBP2 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Accession Number | P52298 |
| Gene Id | 22916 |
| Gene Symbols | NCBP2 |
| Protein Name / Synonyms | NCBP2, CBC2, CBP20, NIP1, PIG55, nuclear cap-binding protein subunit 2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
The product of this gene is a component of the nuclear cap-binding protein complex (CBC), which binds to the monomethylated 5' cap of nascent pre-mRNA in the nucleoplasm. The encoded protein has an RNP domain commonly found in RNA binding proteins, and contains the cap-binding activity. The CBC promotes pre-mRNA splicing, 3'-end processing, RNA nuclear export, and nonsense-mediated mRNA decay. Multiple transcript variants encoding different isoforms have been found for this gene.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:100
IHC 1:50 - 1:200
IF 1:50 - 1:100
Format
Calculated MW
11kDa/15kDa/18kDa
Observed MW
14kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-156 of human NCBP2 (NP_031388.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using NCBP2 antibody (144-07293) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human colon using NCBP2 antibody (144-07293) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human prostate using NCBP2 antibody (144-07293) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of MCF-7 cells using NCBP2 antibody (144-07293). Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.