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Anti-NCF1 Antibody
Catalog #: 144-01148
NCF1 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P14598 |
| Gene Id | 653361 |
| Gene Symbols | NCF1 |
| Protein Name / Synonyms | NCF1, NCF1A, NOXO2, SH3PXD1A, p47phox, neutrophil cytosol factor 1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IP |
| Research Area | Inflammation |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
The protein encoded by this gene is a 47 kDa cytosolic subunit of neutrophil NADPH oxidase. This oxidase is a multicomponent enzyme that is activated to produce superoxide anion. Mutations in this gene have been associated with chronic granulomatous disease.
Dilutions
WB 1:500 - 1:2000
IP 1:50 - 1:100
IP 1:50 - 1:100
Format
Calculated MW
30kDa/44kDa
Observed MW
45kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-390 of human NCF1 (NP_000256.4).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using NCF1 antibody (144-01148) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Immunoprecipitation analysis of 200ug extracts of Raji cells, using 3 ug NCF1 antibody (144-01148). Western blot was performed from the immunoprecipitate using NCF1 antibody (144-01148) at a dilition of 1:1000.
Expiration:
12 months from the date of shipment when stored properly.