Anti-NCF2 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	P19878
Gene Id	4688
Gene Symbols	NCF2
Protein Name / Synonyms	NCF2, NCF-2, NOXA2, P67-PHOX, P67PHOX, neutrophil cytosol factor 2
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC, IF
Research Area	Inflammation
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes neutrophil cytosolic factor 2, the 67-kilodalton cytosolic subunit of the multi-protein NADPH oxidase complex found in neutrophils. This oxidase produces a burst of superoxide which is delivered to the lumen of the neutrophil phagosome. Mutations in this gene, as well as in other NADPH oxidase subunits, can result in chronic granulomatous disease, a disease that causes recurrent infections by catalase-positive organisms. Alternative splicing results in multiple transcript variants encoding different isoforms.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200

Format

Calculated MW

47kDa/50kDa/54kDa/59kDa

Observed MW

68kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 227-526 of human NCF2 (NP_000424.2).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using NCF2 antibody (144-01178) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Western blot - NCF2 antibody (144-01178)

Immunohistochemistry of paraffin-embedded mouse heart using NCF2 antibody (144-01178) at dilution of 1:100 (40x lens).
Immunohistochemistry - NCF2 antibody (144-01178)

Immunofluorescence analysis of HeLa cells using NCF2 antibody (144-01178). Blue: DAPI for nuclear staining.
Immunofluorescence - NCF2 antibody (144-01178)

Expiration:

12 months from the date of shipment when stored properly.

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