Anti-NCK1 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	P16333
Gene Id	4690
Gene Symbols	NCK1
Protein Name / Synonyms	NCK1, NCK, NCKalpha, nck-1, NCK adaptor protein 1
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IF
Shipping Type	Blue ice
Storage	-20°C

Description

Background

The protein encoded by this gene is one of the signaling and transforming proteins containing Src homology 2 and 3 (SH2 and SH3) domains. It is located in the cytoplasm and is an adaptor protein involved in transducing signals from receptor tyrosine kinases to downstream signal recipients such as RAS. Alternatively spliced transcript variants encoding different isoforms have been found.

Dilutions

WB 1:500 - 1:2000
IF 1:50 - 1:200

Format

Calculated MW

35kDa/42kDa

Observed MW

45kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 58-377 of human NCK1 (NP_006144.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of mouse brain, using NCK1 antibody (144-00999) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Western blot - NCK1 Polyclonal Antibody (144-00999)

Immunofluorescence analysis of A549 cells using NCK1 antibody (144-00999). Blue: DAPI for nuclear staining.
Immunofluorescence - NCK1 Polyclonal Antibody (144-00999)

Expiration:

12 months from the date of shipment when stored properly.

Featured Products