Anti-NCK2 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	O43639
Gene Id	8440
Gene Symbols	NCK2
Protein Name / Synonyms	NCK2, GRB4, NCKbeta, NCK adaptor protein 2
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes a member of the NCK family of adaptor proteins. The protein contains three SH3 domains and one SH2 domain. The protein has no known catalytic function but has been shown to bind and recruit various proteins involved in the regulation of receptor protein tyrosine kinases. It is through these regulatory activities that this protein is believed to be involved in cytoskeletal reorganization. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200

Format

Calculated MW

42kDa

Observed MW

43kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-380 of human NCK2 (NP_003572.2).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using NCK2 antibody (144-07750) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.
Western blot - NCK2 antibody (144-07750)

Immunohistochemistry of paraffin-embedded human prostate using NCK2 antibody (144-07750) at dilution of 1:100 (40x lens).
Immunohistochemistry - NCK2 antibody (144-07750)

Expiration:

12 months from the date of shipment when stored properly.

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