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Anti-NCL Antibody
Catalog #: 144-05904
NCL Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Accession Number | P19338 |
| Gene Id | 4691 |
| Gene Symbols | NCL |
| Protein Name / Synonyms | NCL, C23, Nsr1, nucleolin |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Research Area | Angiogenesis |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IHC 1:50 - 1:200
Format
Calculated MW
76kDa
Observed MW
110kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 461-710 of human NCL (NP_005372.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using NCL antibody (144-05904) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Immunohistochemistry of paraffin-embedded human gastric using NCL antibody (144-05904) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using NCL antibody (144-05904) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using NCL antibody (144-05904) at dilution of 1:200 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.