RayBiotech

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ISO 13485:2016 cGMP

Anti-NDEL1 Antibody

Catalog #: 144-05776

NDEL1 Polyclonal Antibody

Product Description

Specifications

Size20 µL, 100 µL
Estimated Lead Time1-2 weeks
SpeciesHuman, Mouse
Host SpeciesRabbit
Accession Number
Q9GZM8
Gene Id
81565
Gene Symbols
NDEL1
Protein Name / Synonyms
NDEL1, EOPA, MITAP1, NDE1L1, NDE2, NUDEL, nudE neurodevelopment protein 1 like 1
ClonalityPolyclonal
IsotypeIgG
Purity
Affinity purification
BuffersPBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended ApplicationsWestern Blotting, IF
Shipping TypeBlue ice
Storage-20°C

Description

Background

This gene encodes a coiled-coil protein that plays a role in multiple processes including cytoskeletal organization, cell signaling and neuron migration, outgrowth and maintenance. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome X.

Dilutions

WB 1:500 - 1:2000
IF 1:50 - 1:100

Format

Calculated MW
37kDa/38kDa/40kDa
Observed MW
39kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-345 of human NDEL1 (NP_110435.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using NDEL1 antibody (144-05776) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Western blot - NDEL1 Polyclonal Antibody (144-05776)

Immunofluorescence analysis of U2OS cells using NDEL1 antibody (144-05776). Blue: DAPI for nuclear staining.
Immunofluorescence - NDEL1 Polyclonal Antibody (144-05776)

Expiration:
12 months from the date of shipment when stored properly.