Anti-NDUFA13 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	Q9P0J0
Gene Id	51079
Gene Symbols	NDUFA13
Protein Name / Synonyms	NDUFA13, B16.6, CDA016, CGI-39, GRIM-19, GRIM19, NADH:ubiquinone oxidoreductase subunit A13
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting
Research Area	Apoptosis, Cardiovascular Disease, Cancer
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes a subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), which functions in the transfer of electrons from NADH to the respiratory chain. The protein is required for complex I assembly and electron transfer activity. The protein binds the signal transducers and activators of transcription 3 (STAT3) transcription factor, and can function as a tumor suppressor. The human protein purified from mitochondria migrates at approximately 16 kDa. Transcripts originating from an upstream promoter and capable of expressing a protein with a longer N-terminus have been found, but their biological validity has not been determined.

Dilutions

WB 1:500 - 1:2000

Format

Calculated MW

16kDa/24kDa

Observed MW

17kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 52-144 of human NDUFA13 (NP_057049.5).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using NDUFA13 antibody (144-05412) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Western blot - NDUFA13 antibody (144-05412)

Expiration:

12 months from the date of shipment when stored properly.

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