[KO Validated] NDUFA7 Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | O95182 |
| Gene Id | 4701 |
| Gene Symbols | NDUFA7 |
| Protein Name / Synonyms | NDUFA7, B14.5a, CI-B14.5a, NADH:ubiquinone oxidoreductase subunit A7 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
This gene encodes a subunit of NADH:ubiquinone oxidoreductase (complex I), which is a multiprotein complex located in the inner mitochondrial membrane. Complex I functions in the transfer of electrons from NADH to the respiratory chain.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:100
IHC 1:50 - 1:100
Format
Calculated MW
12kDa
Observed MW
13kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-113 of human NDUFA7 (NP_004992.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using NDUFA7 antibody (144-08441) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.![Western blot - [KO Validated] NDUFA7 Polyclonal Antibody (144-08441)](https://cdn.sanity.io/images/g51potcz/production/cf4f5bbb122d1de7c9003e13792cbc43ef613d89-588x350.jpg)
Western blot analysis of extracts of various cells, using NDUFA7 antibody (144-08441) at 1:1000 dilution ratio through one-step method.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.![Western blot - [KO Validated] NDUFA7 Polyclonal Antibody (144-08441)](https://cdn.sanity.io/images/g51potcz/production/11a98c4df7699d46eab3bd86e7c42843feedaeee-350x350.jpg)
Western blot analysis of extracts from normal (control) and NDUFA7 knockout (KO) 293T cells, using NDUFA7 antibody (144-08441) at 1:1000 dilution.![Western blot - [KO Validated] NDUFA7 Polyclonal Antibody (144-08441)](https://www.raybiotech.com/files/images/Antibodies/144/144-08441/3.jpg)
Expiration:
12 months from the date of shipment when stored properly.