Anti-NDUFS3 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	O75489
Gene Id	4722
Gene Symbols	NDUFS3
Protein Name / Synonyms	NDUFS3, CI-30, NADH:ubiquinone oxidoreductase core subunit S3
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC
Research Area	Cardiovascular Disease
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes one of the iron-sulfur protein (IP) components of mitochondrial NADH:ubiquinone oxidoreductase (complex I). Mutations in this gene are associated with Leigh syndrome resulting from mitochondrial complex I deficiency.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:100

Format

Calculated MW

14kDa/30kDa

Observed MW

30kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 37-264 of human NDUFS3 (NP_004542.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using NDUFS3 antibody (144-08013) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Western blot - [KO Validated] NDUFS3 Polyclonal Antibody (144-08013)

Western blot analysis of extracts from normal (control) and NDUFS3 knockout (KO) 293T cells, using NDUFS3 antibody (144-08013) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.
Western blot - [KO Validated] NDUFS3 Polyclonal Antibody (144-08013)

Immunohistochemistry of paraffin-embedded human liver injury using NDUFS3 antibody (144-08013) at dilution of 1:100 (40x lens).
Immunohistochemistry - [KO Validated] NDUFS3 Polyclonal Antibody (144-08013)

Immunohistochemistry of paraffin-embedded rat brain using NDUFS3 antibody (144-08013) at dilution of 1:100 (40x lens).
Immunohistochemistry - [KO Validated] NDUFS3 Polyclonal Antibody (144-08013)

Expiration:

12 months from the date of shipment when stored properly.

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